Modifying Enzymes
GeneCopoeia BIOTIN PROTEIN LIGASE
Biotin Protein Ligase - 40 ug: 2 vials of 20 ug each (200,000 Units)
Cayman Chemical Coenzyme AlIthIM salt hydr
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the CoA biosynthetic pathway has been identified as a target for the development of antibacterial compounds
BPS Bioscience Inc Turbo Nuclease, 250 units/μl, recombinant, ≥99% purity, 27 kDa, formulated with 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂, 50% glycerol
Turbo Nuclease, 250 units/μl, recombinant, ≥99% purity, 27 kDa, formulated with 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂, 50% glycerol
Medchemexpress LLC Elastase, Porcine pancreas | 39445-21-1 | 25898.13 | 10 MG
Elastase, Porcine pancreas is a serine protease derived from pig pancreas, composed of a single polypeptide chain of 240 amino acid residues. It functions by hydrolyzing proteins and polypeptides, and research indicates it can induce emphysema in hamsters.
- Derived from pig pancreas
- Serine protease
- Hydrolyzes proteins and polypeptides
- Suitable for research applications only
- Appears as a solid, white to off-white substance
- Store at -20°C for up to 2 years
- Ships at room temperature (within continental US)
Biotium Double Stranded DNA dsDNA121-3 Biotin conjugate 0.1mgmL
Double Stranded DNA dsDNA121-3 Biotin conjugate 0.1mgmL
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. Endoglycosidase Reaction Buffer Pack – 4 x 1 ml
The Endoglycosidase Reaction Buffer Pack contains four buffers: 10X Glycobuffer 2, 10X Glycobuffer 3, 10X Glycoprotein Denaturing Buffer and 10% NP-40, most commonly used in endoglycosidase reactions.
New England Biolabs, Inc. Exonuclease III (E.coli) – 25000 units
Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules. The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Exonuclease III has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities.
New England Biolabs, Inc. NcoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. DNA Polymerase I (E. coli) – 500 units
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3' to 5' and 5' to 3' exonuclease activities. The 5' to 3' exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
New England Biolabs, Inc. Taq DNA Ligase – 2000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).
New England Biolabs, Inc. DNA Polymerase I, Large (Klenow) Fragment – 1000 units
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.